D. Scott Samuels ( edit )

Professor

Contact D. Scott Samuels

Phone: (406) 243-6145
E-mail Address: Scott.Samuels@umontana.edu
Office : Charles H. Clapp 207
Office Hours: By appointment.

Education

B.A. Colorado College, 1983

Ph.D. University of Arizona, 1991

Research Interests

Research in our laboratory focuses on the spirochete Borrelia burgdorferi, the etiologic agent of Lyme disease. Its enzootic life cycle involves a tick vector and a mammalian host: different sets of genes are expressed in these diverse environments. We are interested in the mechanisms by which B. burgdorferi senses that its tick vector is feeding on a vertebrate host and subsequently regulates its gene expression to effect transmission.

Our goal is to dissect the role of small RNAs, RNA-binding proteins, alternative sigma factors, DNA-binding proteins, and DNA supercoiling in the regulation of transcription. We are also interested in the molecular physiology of B. burgdorferi because of its unusual bacterial genome, in particular biogenesis of its ribosomal RNAs and replication of its linear DNA molecules. We have used inhibitors that target DNA gyrase and topoisomerase IV to probe the function of DNA topology in cell growth and gene expression. In addition, we have examined the mechanism of resistance to these and other antibiotics. Furthermore, we have been involved in developing molecular genetic tools to address these research questions, including a system to artificially regulate gene expression in B. burgdorferi in an enzootic cycle model.

Publications

Knight, S.W. and Samuels, D.S. (1999) Natural synthesis of a DNA-binding protein from the C-terminal domain of DNA gyrase A in Borrelia burgdorferi. EMBO J. 18:4875-4881.

Eggers, C.H., Kimmel, B.J., Bono, J.L., Elias, A., Rosa, P., and Samuels, D.S. (2001) Transduction by øBB-1, a bacteriophage of Borrelia burgdorferi. J. Bacteriol. 183:4771-4778.

Alverson, J., Bundle, S.F., Sohaskey, C.D., Lybecker, M.C., and Samuels, D.S. (2003) Transcriptional regulation of the ospAB and ospC promoters from Borrelia burgdorferi. Mol. Microbiol. 48:1665-1677.

Galbraith, K.M., Ng, A.C., Eggers, B.J., Kuchel, C.R., Eggers, C.H., and Samuels, D.S. (2005) parC mutations in fluoroquinolone-resistant Borrelia burgdorferi. Antimicrob. Agents Chemother. 49: 4354-4357.

Gilbert, M.A., Morton, E.A., Bundle, S.F., and Samuels, D.S. (2007) Artificial regulation of ospC expression in Borrelia burgdorferi. Mol. Microbiol. 63: 1259-1273.

Lybecker, M.C., Abel, C.A., Feig, A.L., and Samuels, D.S. (2010) Identification and function of the RNA chaperone Hfq in the Lyme disease spirochete Borrelia burgdorferi. Mol. Microbiol. 78: 622-635.

Hoon-Hanks, L.L., Morton, E.A., Lybecker, M.C., Battisti, J.M., Samuels, D.S., and Drecktrah, D. (2012) Borrelia burgdorferi malQ mutants utilize disaccharides and traverse the enzootic cycle. FEMS Immunol. Med. Microbiol. 66: 157-165.

Drecktrah, D., Hall, L.S., Hoon-Hanks, L.L., and Samuels, D.S. (2013) An inverted repeat in the ospC operator is required for induction in Borrelia burgdorferi. PLoS One 8: e68799.

Field of Study

Molecular borreliology

Courses Taught

BIOB 260 Cellular and Molecular Biology

BCH 110 Introductory Biology for Biochemists