Phone: (406) 243-4834
E-mail Address: frank.rosenzweig@mso.umt.edu
Office : Health Sciences 416The overall goal of research in the Rosenzweig lab is to elucidate mechanisms that produce and maintain diversity in prokaryotic and eukaryotic microbes. We are particularly interested in understanding how changes in genome architecture affect global patterns of gene expression and whether such changes translate into how clones behave physiologically and demographically. We are engaged in several projects related to this theme: we are investigating how yeast and bacterial genomes, including those of interspecific hybrids, respond to either chronic resource limitation or continuously changing temperature and ethanol regimes. We are now also studying how genome architecture of opportunistic pathogens evolves in the cystic fibrosis lung. In the lab and in nature it is possible to track evolution of clonal microbial populations for hundreds of generations. A living record of each population’s evolutionary trajectory can be preserved by archiving samples as -80°C glycerol stocks, and the tempo of evolutionary change inferred from changes in the frequency of neutral markers. The physiology and genetics of evolved strains and their ancestors can then be analyzed in detail to determine the bases for differences in Darwinian fitness. Sequencing of the yeast, E. coli and P. aeruginosa genomes makes possible the use of DNA microarrays to assess how changes in genome architecture and transcript levels underlie, or attend, evolutionary adaptation to various selection pressures.
Kinnersley, M., Holben, W., and F. Rosenzweig. E unibus plurum: Genomic analysis of an experimentally evolved polymorphism in Escherichia coli. PLoS Genetics In Press.
Kruckeberg, A. L., Nagarajan, S., McInnerney, K. and F. Rosenzweig. 2009. Extraction of RNA from Ca-alginate-encapsulated yeast for transcriptional profiling. Analytical Biochem 391(2):160
Rosenzweig, F. and Sherlock, G. 2009. Through a Glass, Clearly: Experimental Evolution as a Window on Adaptive Genome Evolution. In EXPERIMENTAL EVOLUTION: Concepts, Methods, and Applications, Eds. T. Garland, Jr. and M. R. Rose. University of California Press, In press.
Huey, R. and Rosenzweig, F. 2009. Laboratory evolution meets Catch-22: Balancing Simplicity and Realism. In EXPERIMENTAL EVOLUTION: Concepts, Methods, and Applications, Eds. T. Garland, Jr. and M. R. Rose. University of California Press, In press.
Ramamoorthy, S., Piotrowski, J. S., Langner, H. W., Holben, W. E., Morra, M. J. and R. F. Rosenzweig. 2009. Ecology of sulfate-reducing bacteria in an iron-dominated, mining-impacted freshwater sediment. J Environ Quality 38(2):675-684.
Sass, H., Ramamoorthy, S., Yarwood, C., Langner, H., Schumann, P., Kroppenstedt, R. M., Spring, S. and R. F Rosenzweig. 2009. Characterization of novel sulfate-reducing bacteria from a metal(loid)-contaminated freshwater sediment: Description of Desulfovibrio idahonensis sp. nov. Int J Syst Evol Microbiol 59:xxx-xxx.
Ramamoorthy, S., Sass, H., Langner, H., Schumann, P., Kroppenstedt, R. M., Spring, S., Overmann, J., and R. F. Rosenzweig. 2006. Desulfosporosinus lacus sp. nov., a sulfate-reducing bacterium isolated from pristine freshwater lake sediments. Int J Syst Evol Microbiol 56: 2729-2736
Spring, S. and R.F. Rosenzweig. 2004. The Genera Desulfitobacterium and Desulfosporosinus: Taxonomy. In, THE PROKARYOTES, M. Dworkin, (ed.) Springer-Verlag, New York.
Cummings, D.E., Snoeyenbos-West, O., Newby, D. T., Niggemyer, A. M., Lovley, D. R., Achenbach, L. A. and R. F. Rosenzweig. 2003. Diversity of Geobacteraceae species inhabiting metal-polluted freshwater lake sediments ascertained by 16S rDNA analyses. Microbial Ecology. 46:257-269
Nicholas, D. R., Ramamoorthy, S., Spring, S., Moore, J. N., and R. F. Rosenzweig. 2003. Biogeochemical transformations of arsenic in circumneutral freshwater sediments. Biodegradation 14: 123-137.
Dunham, M. J., Badrane, H., Ferea, T., Adams, J. P., Brown, P. O., Rosenzweig, R. F. and D. Botstein. 2002. Characteristic genome rearrangements accompany experimental evolution of S. cerevisiae. PNAS (USA). 99(25):16144-16149.
Laub, M. and F. Rosenzweig. 2002. Transcriptional profiling in bacteria using microarrays. In Methods and Tools in Biosciences and Medicine: Prokaryotic Genomics and Genetics, Edited by M. Blot. Birkhauser Verlag, Basel.
Niggemyer, A., Spring, S., Stackebrandt, E. and R. F. Rosenzweig. 2001. Isolation and characterization of a novel As(V) reducing bacterium: Implications for arsenic mobilization and the genus Desulfitobacterium. Appl. Env. Microbiol. 67: 5568-5580.
Cummings, D. E., Spring, S. and R. F. Rosenzweig. 2001. The ecology of iron-reducing bacteria in natural and affected environments. Manual of Environmental Microbiology, 2nd Edition (ASM Press).
Wielinga, B., Bostick, B., Rosenzweig, R. F. and S. Fendorf. 2000. Inhibition of bacterially promoted uranium reduction: Ferric (hydr)oxides as competitive electron acceptors. Environ. Sci. Technol. 34:2190-2195.
Cummings, D. E., March, A. W., Bostick, B., Spring, S., Caccavo, F., Fendorf, S. E. and R. F. Rosenzweig. 2000. Evidence for microbial Fe(III) reduction in anoxic, mining- impacted lake sediments (Lake Coeur d’Alene, USA). Appl Env Microbiol 66: 154-162.
Ferea, T. L., Botstein, D., Brown, P. O., Rosenzweig R. F. 1999. Systematic changes in gene expression patterns following adaptive evolution in yeast. Proc Natl Acad Sci U S A. 96:9721-9726.
Cummings, D. E, Caccavo, F., Spring, S., and R. F. Rosenzweig. 1999. Ferribacterium limneticum gen. Nov., sp. Nov., an Fe(III)-reducing microorganism isolated from mining-impacted freshwater lake sediments. Archives Microbiology 171:183-188.
LaForce, M. J., Fendorf, S. E., Li, G. C., and R. F. Rosenzweig. 1999. Redistribution of trace elements from contaminated sediments of Lake Coeur d'Alene during oxygenation. J. Environ. Quality. 28:1195-1200.
Cummings, D. E., Caccavo, F., Fendorf, S. E. and R. F. Rosenzweig. 1999. Arsenic mobilization by the dissimilatory Fe(III)-reducing bacterium Shewanella alga BrY. Environ Sci Technol 33:723-729.
Harrington, J. M., Fendorf, S. E., Wielinga, B. W. and R. F. Rosenzweig. 1999. Response to comments on "Phase associations and mobilization of iron and trace metals in sediments of Lake Coeur d'Alene, ID." Environ Sci Technol. 33: 203-204.
Harrington, J. M., Fendorf, S. E. and R. F. Rosenzweig. 1998. Biotic generation of arsenic (III) in heavy metal contaminated lake sediments. Environ Sci Technol 32:2425-2430.
Brown, C. J., Todd, K. M. and R. F. Rosenzweig. 1998. Multiple duplications of yeast hexose transport genes in response to selection in a glucose-limited environment. Mol Biol Evoln 15:931-942.
Harrington, J. M., LaForce, M. J., Rember, W. C., Fendorf, S. E. and R. F. Rosenzweig. 1998. Phase associations and mobilization of iron and trace metals in sediments of Lake Coeur d’Alene, ID Environ Sci Technol 32:650-666.
LaForce, M. J., Fendorf, S. E., Li, G. C., Schneider, G. M. and R. F. Rosenzweig. 1998. A laboratory evaluation of trace element mobility from flooding and nutrient loading of Coeur d’Alene River sediments. J Environ Quality 27:318-328.
Rosenzweig, R. F., Treves, D., Sharp, R., and J. Adams. 1994. Microbial evolution in a simple unstructured environment: genetic differentiation in Escherichia coli. Genetics 137:903-917.
Rosenzweig, R. F. and J. Adams. 1994. Microbial adaptation to a changeable environment cell-cell interactions mediate physiological and genetic differentiation. Bioessays 16:715-717.
BIOL100N – The Science of Life
MICB404 – Microbial Genetics
MICB405 – Experimental Microbial Genetics
BIOL497 – Advanced Undergraduate Research
