Phone: 406-243-6393
E-mail Address: stephen.lodmell@umontana.edu
Office : Chcb 202
Office Hours: Office Hours Spring 09:
M, W, F 11:10-12:00
and by appointment
A group open discussion session is offered every Thursday 5-6pm in CHCB230.
---My research interests lie in the mechanisms of molecular recognition between biological macromolecules. In particular, I am interested in RNA-RNA and RNA-protein interactions in two different systems: those that are essential for specific replicative functions in retroviruses, and those that are important for ribosome function. The genomic RNA of HIV-2 contains not only coding information for proteins, but also functional signals for transactivation, dimerization, encapsidation, splicing, and poly A addition. Thus one major effort in my laboratory is to construct detailed secondary and tertiary structure maps of HIV-2 RNA functional motifs using solution structure probing techniques.
The ultimate goal of this line of research is to understand the structure and functions of this crucial portion of the HIV genome in sufficient detail to be able to target it for antiretroviral chemotherapies. The other major thrust in our laboratory is using novel tethered chemical nucleases and crosslinking agents to investigate structural dynamics of the ribosome during protein synthesis. The high resolution crystal structures of the ribosome has recently been published which has given us a good look at the static structure of these universal subcellular particles. However, we know that the ribosome is quite dynamic during the processive catalysis of peptide bond formation, thus our efforts are focused on elucidating the important conformational changes that occur in the ribosome during protein synthesis.
Baig TT, Lanchy JM, Lodmell JS (2009) Randomization and in vivo selection reveal a GGRG motif essential for packaging human immunodeficiency virus type 2 RNA. J Virol 83: 802-810.
Baig, T. T., C. L. Strong, J. S. Lodmell, and J. M. Lanchy. 2008. Regulation of primate lentiviral RNA dimerization by structural entrapment. Retrovirology 5:65.
Lanchy, J.M. and Lodmell, J.S. (2007) An extended stem-loop 1 is necessary for human immunodeficiency virus type 2 replication and affects genomic RNA encapsidation. J Virol, 81, 3285-3292.
Baig, T.T., Lanchy, J.M. and Lodmell, J.S. (2007) HIV-2 RNA dimerization is regulated by intramolecular interactions in vitro. RNA, 13, 1341-1354.
Fabbretti, A., Pon, C.L., Hennelly, S.P., Hill, W.E., Lodmell, J.S. and Gualerzi, C.O. (2007) The real-time path of translation factor IF3 onto and off the ribosome. Mol Cell, 25, 285-296.
Criswell, D., Tobiason, V.L., Lodmell, J.S. and Samuels, D.S. (2006) Mutations conferring aminoglycoside and spectinomycin resistance in Borrelia burgdorferi. Antimicrob Agents Chemother, 50, 445-452.
S.P. Hennelly, A. Antoun, M. Ehrenberg, C.O. Gualerzi, W. Knight, J.S. Lodmell, and W.E. Hill, (2005) A Time-resolved Investigation of Ribosomal Subunit Association. J Mol Biol, 346, 1243-1258.
J.-M. Lanchy, Q.N. Szafran, J.S. Lodmell (2004) Splicing affects presentation of RNA dimerization and encapsidation signals in vitro. Nucleic Acids Research 32:4585-4595.
J.-M. Lanchy, J.D. Ivanovitch, J.S. Lodmell (2003) A structural linkage between dimerization and encapsidation signals in HIV-2 RNA in vitro. RNA 9(8): 1007-1018.
W.S. Bowen, N. Van Dyke, E.J. Murgola, J.S. Lodmell, and W.E. Hill (2005) Interaction of thiostrepton and elongation factor-G with the ribosomal protein L11-binding domain. J Biol Chem, 280, 2934-2943.
J.-M. Lanchy, C.A. Rentz, J.D. Ivanovitch, and J.S. Lodmell. (2003) Elements located upstream and downstream of the major splice donor site influence the ability of HIV-2 leader RNA to dimerize in vitro. Biochemistry 42: 2634-2642.
S. Marzi, W. Knight, L. Brandi, E. Caserta, N. Soboleva, W.E. Hill, C.O. Gualerzi, and J.S. Lodmell (2003) Ribosomal localization of translation initiation factor IF2. RNA 9(8): 958-969.
J.-M. Lanchy, J.D. Ivanovitch, J.S. Lodmell (2003) A structural linkage between dimerization and encapsidation signals in HIV-2 RNA in vitro. RNA 9(8): 1007-1018.
J.-M. Lanchy, C.A. Rentz, J.D. Ivanovitch, and J.S. Lodmell. (2003) Elements located upstream and downstream of the major splice donor site influence the ability of HIV-2 leader RNA to dimerize in vitro. Biochemistry 42: 2634-2642.
E. L. Deer, B. Douk, J.-M. Lanchy, and J.S. Lodmell. (2003) Elucidation and characterization of oligonucleotide-accessible sites on HIV-2 leader region RNA. Antisense and Nucleic Acid Drug Development 13: 45-55.
J.-M. Lanchy and J.S. Lodmell. (2002) Alternate usage of two dimerization sites in HIV-2 viral RNA in vitro. J. Mol. Biol. 319:637-648.
J.S.Lodmell & S.P. Hennelly (2002). "Conformational Dynamics Within the Ribosome." In Translational Mechanisms (Brakier-Gingras, L. & Lapointe, J., eds.). Landes Bioscience, Austin, TX.
W.S. Bowen, W.E. Hill, and J.S. Lodmell (2001). "Comparison of rRNA cleavage by complementary 1,10-phenanthroline-Cu(II)- and EDTA-Fe(II)- derivatized oligonucleotides". Methods 25: 344-350.
J.S. Lodmell, C. Ehresmann, B. Ehresmann, and R. Marquet (2001). “Structure and dimerization of RNA kissing loop aptamers.” J. Mol. Biol. 311: 475-490.
