Covalent Modification of Glutamate
- Other irreversible inhibitors have particular structures that allow them to bind specifically
to the active site of an enzyme
- Bromohydroxyacetone phosphate (BHAP) is an analog of dihydroxyacetone phosphate (DHAP), which binds
to the active site of triose phosphate isomerase (an enzyme we'll see later in glycolysis)
- In the reaction, the bromine group on the inhibitor leaves, resulting in the formation of
a covalent bond to the active Glu residue on the enzyme