Lineweaver-Burk Plot
- This double-reciprocal plot is also called the Lineweaver-Burk plot. Because it is a straight
line, the curve is represented by the general formula y = mx + b, where m is the slope and b is the y intercept
- Here, we see from the equation that the y intercept is 1 / Vmax. If we set y = 0 and solve
for x, we find that the x intercept is equal to -1/Km
- Although computers have made the experimental use of Lineweaver-Burk plots unnecessary, they are still
useful for representing changes in Vmax and Km, as we will see in the subsequent sections on
enzyme inhibition