| Reagent | Cleavage Location
| Cyanogen bromide | carbonyl side of methionine residues
| Trypsin | carbonyl side of lysine and arginine residues
| V8 protease | carbonyl side of aspartate and glutamate residues
| Chymotrypsin | carbonyl side of bulky hydrophobic residues such as phenylalanine, tyrosine and tryptophan
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